Stem Cells Applications in Diseases by Mikkel L. Sorensen

By Mikkel L. Sorensen

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By Mikkel L. Sorensen

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2002). An expanding universe of noncoding RNAs. Science, 296, 1260– 1263. F. (2005). Mapping of conserved RNA secondary structures predicts thousands of functional noncoding RNAs in the human genome. Nat. , 23, 1383–1390. , and Szostak JW. (1990). In vitro selection of RNA molecules that bind specific ligands. Nature, 346, 818–822. , and Gold, L. (1990). Systemic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase. Science, 249, 505– 510. A. (2002). RNAi related mechanisms affect both transcriptional and posttranscriptional transgene silencing in Drosophila.

1995): Survival and differentiation of adult neuronal progenitor cells transplanted to the adult brain. Proc. Natl. Acad. Sci. USA, 92:11879–83. Nait-Oumesmar B, Decker L, Lachapelle F, Avellana-Adalid V, Bachelin C, Van Evercooren AB. (1999): Progenitor cells of the adult mouse subventricular zone proliferate, migrate and differentiate into oligodendrocytes after demyelination. Eur. J. , 11:4357–66. Nakayama T, Sai T, Otsu M, Momoki-Soga T, Inoue N. (2006): Astrocytogenesis of embryonic stem-cell-derived neural stem cells: Default differentiation.

Further cultivation in ACM resulted in complete progression of neurogenesis of neural stem cells. To suppress neurogenesis and promote the proliferation of neural stem cells, culture medium was changed from ACM to Neurobasal B-27 supplemented with FGF-2 after attachment of the NSS to the adhesive substrate [7]. Following the switch in culture medium, many of the neural stem cells migrated from the attached NSS to the surrounding area forming a circular monolayer (Figure 1, C). After one week, the circular area of monolayer culture had spread 300–500 μm in diameter and included more than 100,000 neural stem cells.

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