By Zdenek Deyl
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Additional resources for Electrophoresis
Sample text
On substituting E = - U y L-' and x = Xy-' (where U is the total voltage applied, L the length of the porous medium, y the tortuosity factor, x the real and X the macroscopically measured distance to which the ith compound travelled during time t ) in eqn. 45) is obtained. It appears that for a porous medium containing pores with a diameter larger than that of any of the separated particles, the distribution function determined in this way is identical with the adsorption isotherm obtained when using static methods.
Calculation for zone electrophoresis . . . . . . . . . . . . . . . . . . . . . . . Calculation for the moving boundary method. Calculation for isotachophoresis . . . . . . . . . . . . . . . . . . . . . . . . Calculation for focusing methods . . . . . . . . . . . . . . . . . . . . . . . . Principles of quantitative evaluation. 39 40 40 41 41 42 42 INTRODUCTION The objectives of electrophoretic separations may differ.
Calculation for focusing methods . . . . . . . . . . . . . . . . . . . . . . . . Principles of quantitative evaluation. 39 40 40 41 41 42 42 INTRODUCTION The objectives of electrophoretic separations may differ. Preparative electrophoresis is used for separation of individual substances from a mixture, and these substances are further identified and determined. When using electromigration methods for analytical purposes, the purpose is to characterize qualitatively and quantitatively the components of a separated mixture.