Methods in Bioengineering: Alternative Technologies to by Tim Maguire, Eric Novik

By Tim Maguire, Eric Novik

Written and edited by means of famous specialists within the box, the hot "Artech residence equipment in Bioengineering" e-book sequence bargains unique suggestions on authoritative equipment for addressing particular bioengineering demanding situations. providing a hugely functional presentation of every subject, each one e-book presents examine engineers, scientists, and scholars with step by step techniques, transparent examples, and powerful how one can conquer difficulties which may be encountered. offering possible choices to animal checking out is likely one of the preferred themes in biomedical examine, and this groundbreaking quantity addresses this severe matters head on. This specific e-book offers options and techniques on the vanguard of clinical examine that experience the aptitude to exchange definite entire animal assessments. additionally, this booklet presents a platform the place different largely authorized ideas and clinical developments will be collated right into a concise set of tools that may be carried out inside of either educational and commercial groups.

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By Tim Maguire, Eric Novik

Written and edited by means of famous specialists within the box, the hot "Artech residence equipment in Bioengineering" e-book sequence bargains unique suggestions on authoritative equipment for addressing particular bioengineering demanding situations. providing a hugely functional presentation of every subject, each one e-book presents examine engineers, scientists, and scholars with step by step techniques, transparent examples, and powerful how one can conquer difficulties which may be encountered. offering possible choices to animal checking out is likely one of the preferred themes in biomedical examine, and this groundbreaking quantity addresses this severe matters head on. This specific e-book offers options and techniques on the vanguard of clinical examine that experience the aptitude to exchange definite entire animal assessments. additionally, this booklet presents a platform the place different largely authorized ideas and clinical developments will be collated right into a concise set of tools that may be carried out inside of either educational and commercial groups.

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6, No. 4, 2003, pp. 481–485. Obach, R. , “The Prediction of Human Pharmacokinetic Parameters from Preclinical and In Vitro Metabolism Data,” J. Pharmacol. Exp. , Vol. 283, No. 1, 1997, pp. 46–58. Obach, R. , “Prediction of Human Clearance of Twenty-Nine Drugs from Hepatic Microsomal Intrinsic Clearance Data: An Examination of In Vitro Half-Life Approach and Nonspecific Binding to Microsomes,” Drug Metab. , Vol. 27, No. 11, 1999, pp. 1350–1359. , H. Takahashi, and Y. Ishii, “A Convenient In Vitro Screening Method for Predicting In Vivo Drug Metabolic Clearance Using Isolated Hepatocytes Suspended in Serum,” Drug Metab.

2-μm filter membrane (Thermo Scientific Nalgene MF75 Series Sterile Disposable Tissue Culture Filter Units—Part # 09-741-02) and store at 4°C before use. Use the medium within 6 months. Seed and subculture cells 1. Thaw one or two frozen vials by rapid agitation in a 37°C water bath. 2. Immediately after the cell suspension is melted, remove it from the water bath and transfer the cell suspension from the vial to a 225 cm2 culture flask, which contains a 40-mL culture medium using a serological pipette.

3, No. 4, 2009, pp. 296–307. , “Enhanced Maintenance and Functions of Rat Hepatocytes Induced by Combination of On-Site Oxygenation and Coculture with Fibroblasts,” J. , Vol. 133, No. 2, 2008, pp. 253–260. Khetani, S. , and S. N. Bhatia, “Microscale Culture of Human Liver Cells for Drug Development,” Nat. , Vol. 26, No. 1, 2008, pp. 120–126. Shackman, J. , “Perfusion and Chemical Monitoring of Living Cells on a Microfluidic Chip,” Lab Chip, Vol. 5, No. 1, 2005, pp. 56–63. , “An Integrated Microfluidic System for Long-Term Perfusion Culture and On-Line Monitoring of Intestinal Tissue Models,” Lab Chip, Vol.

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