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Extra info for Molecular Cloning - Selected Applns. in Medicine and Biology
Sample text
Nevertheless, calcium phosphate nanoparticles introduced by Roy et al. (2003) are ultra-low size entities around 80 nm in diameter and it seems to be able to protect encapsulated DNA from environmental DNases with capability of surface modifications. This new class of nanoparticles have been used for gene delivery to liver. 2 Diethylaminoethyl (DEAE)-dextran This method is based upon the negative charge of DNA and the positive charge of diethyl aminoethyl-dextran leading to the formation of a complex and adherence of the complex to the cell surface followed by endocytosis.
Gene Vol. 189, pp 159162. Jacobson RH, Zhang XJ, Dubose RF & Matthews BW (1994) Three-dimensional structure of β-galactosidase from E. coli. Nature, Vol. 369, No. 6483, pp 761-766. Katzen F (2007) Gateway recombination cloning: a biological operating system. Expert Opinion in Drug Discovery, Vol . 2, No. 4, pp 571-581 Kemp DJ & Cowman AF (1981) Direct immunoassay for detecting E. coli colonies that contain polypeptides encoded by cloned DNA segments. Proceedings of National Academy of Sciences USA, Vol.
Clifton, New Jersey, United Stated. Gonzalez H, Hwang SJ, Davis ME (1999). New class of polymers for the delivery of macromolecular therapeutics, Bioconjug Chem. 10:1068– 1074. Gulick T (2003). Curr Protoc Cell Biol. Transfection using DEAE-dextran. 4. Guyden J (1993). Techniques for gene cloning and expression. In: Recombinant DNA technology edited by Steinberg M, PTR Printic Hall, New Jersey, United States, pp: 81-97. Hammarskjold ML (1991). Manipulation of SV40 vectors. In: Methods in molecular biology, Vol7: Gene transfer and expression protocols edited by Murray EJ Clifton, Humana Press Inc, pp: 169-173.