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Further cultivation in ACM resulted in complete progression of neurogenesis of neural stem cells. To suppress neurogenesis and promote the proliferation of neural stem cells, culture medium was changed from ACM to Neurobasal B-27 supplemented with FGF-2 after attachment of the NSS to the adhesive substrate . Following the switch in culture medium, many of the neural stem cells migrated from the attached NSS to the surrounding area forming a circular monolayer (Figure 1, C). After one week, the circular area of monolayer culture had spread 300–500 μm in diameter and included more than 100,000 neural stem cells.