By Bernd Sokolowski
This moment version expands upon the former quantity with new and up-to-date chapters. Auditory and Vestibular study: equipment and Protocols, moment Edition courses readers via protocols on mobilephone tradition, tissue engineering, nanotechnology, high-throughput screening, and physiology. Chapters on body structure hide thoughts that come with optical coherence tomography, patch clamping, and photostimulation of caged neurotransmitters. Written within the hugely profitable Methods in Molecular Biology series structure, chapters contain introductions to their respective themes, lists of the mandatory fabrics and reagents, step by step, with ease reproducible laboratory protocols, and tips about troubleshooting and keeping off recognized pitfalls.
Authoritative and cutting-edge, Auditory and Vestibular examine: equipment and Protocols, moment version aims to make sure profitable leads to the extra examine of this very important field.
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Additional info for Auditory and Vestibular Research: Methods and Protocols
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3 Microinjection and Electroporation 31 1. , Novato, CA). 2. Micropipettes: thin wall borosilicate glass capillary with filament, diameter chosen to match electrode holder. 17 mm ID (Harvard Apparatus, Holliston, MA). 3. ), and set on an iron base plate (Narishige, Japan). 4. Electroporator (TSS20 Ovodyne) and current amplifier (E21) (Intracel, UK). 5. , Tritech Research). 6. , Tokyo, Japan). 7. , Cleveland, OH). 8. 5 mm glass OD (Harvard Apparatus). 9. Manual stereomicroscope with apochromatic correction and magnification to 25× or greater.
Org; [32]. It is also possible to construct an intron that carries multiple pre-miR sequences in series [12]. See manufacturer for further details of Gateway technology (Invitrogen, Life Technologies). Plasmids pMEMCS-SDA and pTol2-GFP-Gateway are available from the authors upon request. 2. When making high concentration DNA, we often resuspend in a lower volume (~25–50 %) than what is recommended by the manufacturer of a commercial maxiprep kit. The resulting solution can be rather viscous or DNA may precipitate out of solution.
2. 5–20-Gauge needle. 3. Scotch tape and clear packing tape. 32 Michelle L. Stoller and Donna M. Fekete 4. 5 mL plastic disposable transfer pipets. 5. Egg holder: can be fashioned from Styrofoam, plaster-of-paris, or by lining a 60 mm Petri dish with Kimwipes. 6. Dissection tools: one Dumont #55 straight forceps, one Dumont #5 straight forceps with Biology tips, one set of curved dissection scissors (Fine Science Tools, Foster City, CA). 7. Kimwipes. 8. 70 % Ethanol. 9. , Chula Vista, CA). 1 DNA Plasmid Construction and Preparation 1.