By L. L. Butcher, N. J. Woolf (auth.), Israel Hanin Ph.D. (eds.)
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44) and Hattori et al. (23) had noted positive cortical and medial habenula neurons but uncertainty about distinguishing between cholinergic and intensely cholinoceptive neurons was raised by the results obtained following improvements in the methods of fixation (32, 33). It should be anticipated that some differences in immunohistochemical results will occur between laboratories since particular antibodies may recognize different sites on ChAT, or because the conformation of ChAT may vary according to the method of fixation or its location within the CNS.