By Dan E. Robertson and Joseph P. Noel (Eds.)
This MIE quantity covers tools for a large number of subject matters between that are computational tools, laboratory tools, enzyme optimization, binding proteins/antibodies, and screening applied sciences.
Table of Contents
-Methodology
-Applications-Opzimization and Screening
-Applications-Directed Evolution of Enzymatic Function
-Applications-Evolution of Biosynthetic Pathways
-Devices, Antibodies and Vaccines
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The chimeric enzyme had BCF for xylotetraose more similar to that of CfXyn10A than to that of SoXyn10A. The BCFs of chimera for xylopentaose again showed similar properties to CfXyn10A. [3] module shuffling 33 Fig. 7. Rate of hydrolysis and bond cleavage frequencies of xylooligosaccharides. (A) Rate of xylooligosaccharide hydrolysis catalyzed by CfXyn10A, SoXyn10A, and FC-14-15. (B) Bond cleavage frequencies of xylooligosaccharides catalyzed by CfXyn10A, SoXyn10A, and FC-14-15. SoXyn10A (&), CfXyn10A (d), and FC-14-15 (m) were incubated with xylooligosaccharides of different lengths, and the rate of substrate hydrolysis was used to calculate kcat/Km in A.
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