By by J.F. Patience, P.A. Thacker and C.F.M. de Lange.
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Additional info for Swine Nutrition Guide 2nd Edition
2. 24 Frederick Khachik neurosporene (34) ζ-carotene (38) phytofluene (42) phytoene (44) Fig. 2. ) and 475 nm (___). HPLC conditions. 6 mm. 1%). 7 mL/min. Peak identification. 1: (Z)-ζ-carotene; 2: (E)-ζ-carotene (38); 3: (E)-αcarotene (7); 4: (E)-β-carotene (3); 5: (Z)-β-carotene; 6: nonapreno-β-carotene (1) internal standard. Reproduced from  with permission. Analysis of Carotenoids in Nutritional Studies 25 For a natural extract, carotenes containing various combinations of these structural features may be present, all influencing retention in different ways.
The occurrence and distribution of nutritionally important carotenoids in foods, especially fruits and vegetables, is surveyed in Chapter 3 and the distribution of carotenoids in human blood, organs and tissues is covered in Chapter 7. In the past two decades, technological advances in high-performance liquid chromatography (HPLC) have provided analysts with powerful, sensitive tools to separate carotenoids and low levels of their metabolites and analyse them quantitatively with great precision.
In recent years, investigations have spread in directions as diverse as whole population studies (epidemiology), detailed investigation of effects on molecular processes and intricate mechanistic studies. The literature is vast and expanding rapidly. This, the final volume of the Carotenoids series, surveys the field of carotenoids in human nutrition and health. In the past 15 years or so, the topic has been covered in several books. Two of these, ‘Carotenoids in 2 George Britton, Synnøve Liaaen-Jensen and Hanspeter Pfander Human Health’  and ‘Carotenoids and Retinoids: Molecular Aspects and Health Issues’  comprise collections of papers presented at meetings.