The Wilms’ Tumor (WT1) Gene: Methods and Protocols by Nicholas Hastie

By Nicholas Hastie

This quantity presents a radical review of the Wilms’ Tumour Gene (WT1). The ebook starts with 3 assessment chapters that disguise the involvement of WT1 in pediatric melanoma, kidney sickness, and tissue improvement and homeostasis. the following few chapters talk about mobile marking and lineage tracing, epicardial mobilephone method, colony forming assays for bone marrow stem cells, angiogenesis assays and zebrafish tools.  the following team of chapters explores the newest instruments in genomics, molecular biology, and biochemistry. They speak about dissecting transcription issue functionality in phone loose structures, ChiP seq, proteomics, RNA interactome, and multiphoton imaging of lipids, measuring the binding constants of protein-nucleic acid interactions, and bioinformatics techniques for studying subsequent new release series facts. the ultimate bankruptcy discusses protocols for medical trials for immune remedy utilizing anti-WT1 peptides. Written within the hugely profitable Methods in Molecular Biology sequence structure, chapters contain introductions to their respective themes, lists of the required fabrics and reagents, step by step, effectively reproducible laboratory protocols, and tips about troubleshooting and heading off recognized pitfalls.

Practical and thorough, The Wilms’ Tumour (WT1) Gene: equipment and Protocols is a worthy source for somebody who's attracted to the varied methodologies utilized in WT1 research.

 

Show description

By Nicholas Hastie

This quantity presents a radical review of the Wilms’ Tumour Gene (WT1). The ebook starts with 3 assessment chapters that disguise the involvement of WT1 in pediatric melanoma, kidney sickness, and tissue improvement and homeostasis. the following few chapters talk about mobile marking and lineage tracing, epicardial mobilephone method, colony forming assays for bone marrow stem cells, angiogenesis assays and zebrafish tools.  the following team of chapters explores the newest instruments in genomics, molecular biology, and biochemistry. They speak about dissecting transcription issue functionality in phone loose structures, ChiP seq, proteomics, RNA interactome, and multiphoton imaging of lipids, measuring the binding constants of protein-nucleic acid interactions, and bioinformatics techniques for studying subsequent new release series facts. the ultimate bankruptcy discusses protocols for medical trials for immune remedy utilizing anti-WT1 peptides. Written within the hugely profitable Methods in Molecular Biology sequence structure, chapters contain introductions to their respective themes, lists of the required fabrics and reagents, step by step, effectively reproducible laboratory protocols, and tips about troubleshooting and heading off recognized pitfalls.

Practical and thorough, The Wilms’ Tumour (WT1) Gene: equipment and Protocols is a worthy source for somebody who's attracted to the varied methodologies utilized in WT1 research.

 

Show description

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Extra resources for The Wilms’ Tumor (WT1) Gene: Methods and Protocols

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Cancer Res 60(15):4030–4032 5. Iijima K, Someya T, Ito S et al (2012) Focal segmental glomerulosclerosis in patients with complete deletion of one WT1 allele. Pediatrics 129(6):e1621–e1625 6. Guo JK, Menke AL, Gubler MC et al (2002) WT1 is a key regulator of podocyte function: 20 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. Eve Miller-Hodges reduced expression levels cause crescentic glomerulonephritis and mesangial sclerosis. Hum Mol Genet 11(6):651–659 Menke AL, IJpenberg A, Fleming S, Ross A et al (2003) The wt1-heterozygous mouse; a model to study the development of glomerular sclerosis.

Given the variability of genotype-phenotype correlations, genetic testing also remains vital for other family members when screening for potential organ donors and for the likelihood of disease recurrence in a transplanted graft, and, in theory, for pre-emptive treatment strategies. Although no evidence yet exists for preventative treatment in affected individuals, it is known in other genetic forms of kidney disease that such strategies can be successful, such as the specific use of Angiotensin Converting Enzyme inhibitors in Alport syndrome [32, 33].

Hahn H, Cho YM, Park YS et al (2006) Two cases of isolated diffuse mesangial sclerosis with WT1 mutations. J Korean Med Sci 21(1):160–164 30. Jeanpierre C, Denamur E, Henry I et al (1998) Identification of constitutional WT1 mutations, in patients with isolated diffuse mesangial sclerosis, and analysis of genotype/ phenotype correlations by use of a computerized mutation database. Am J Hum Genet 62(4):824–833 Clinical Aspects of WT1 and the Kidney 31. Brown EJ, Pollak MR, Barua M (2014) Genetic testing for nephrotic syndrome and FSGS in the era of next-generation sequencing.

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