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2001) tested a taurine assay in pet food after acid hydrolysis (6 M HCl, 110°C, 16 h) and precolumn derivatization with dansyl chloride in a collaborative trial. 3% were achieved, and the procedure was adopted as the official method by AOAC International. Porter et al. (1988), Amiss et al. (1990), Sakai and Nagasawa (1992) and Messing and Sturmann (1993) described the determination of taurine in feline and other blood and plasma by HPLC separation following precolumn derivatization with different reagents.
Molnár-Perl (1997) has compiled a review of methods of tryptophan analysis in peptides and proteins, including descriptions of techniques not normally used in feedingstuffs, such as acid or enzymatic hydrolysis, measurement by gas chromatography or derivative spectroscopy whithout chemical derivatization. Carisano (1993), who employed microwave hydrolysis with lithium hydroxide under temperature control, was able to hydrolyse meat and fish completely in less then 60 min, obtaining results similar to those with barium hydroxide (110°C, 12 h).
Due to the complexity and high cost of the technique, chromatographic amino acid analysis is usually not available to the feed manufacturer, but he will seek to improve his matrix data in the medium term by having his own raw materials analysed in an external laboratory. Up-to-date tabular data by national organizations (CVB, 2001; NRC, 1998, 2001) summarizing the amino acid concentrations of raw materials across many samples are also helpful. Fickler et al. (2001) have compiled more than 15,000 amino acid profiles of 120 raw materials which provide information on variations in the concentrations, together with breakdowns by country of origin.