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The severely acclaimed laboratory general, Methods in Enzymology, is without doubt one of the such a lot hugely revered guides within the box of biochemistry. given that 1955, every one quantity has been eagerly awaited, usually consulted, and praised by way of researchers and reviewers alike. The sequence includes a lot fabric nonetheless appropriate this present day - really an important booklet for researchers in all fields of lifestyles sciences
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A. O'Sullivan and N. Sueoka, J. Mol. Biol. 27,349 (1967). [2] EXPERIMENTAL METHODS FOR B a c i l l u s s u b t i l i s 41 TABLE II REPRESENTATIVEBacillus subtilis AMINO ACID AUXOTROPHSa Amino acid requirement Arginine Aromatic (phenylalanine, tyrosine, or tryptophan) Aspartic acid Cysteine Glutamic acid Glycine Histidine Isoleucine, leucine, valine Lysine Methionine Phenylalanine Proline Serine Threonine Tryptophan Tyrosine Source of mutants References y-Irradiation, spores HNO2 UV, X-ray UV, NG in vivo b c d, m e, h X-ray UV, X-ray HNO2 UV, X-ray UV UV, HNO2 Heat, spores UV, X-ray HNO2 UV, X-ray HNO2 UV, X-ray UV, X-ray HNO2 EMS in vivo UV HNO2 HNO2 UV, X-rays UV, X-rays NG in vivo HNO2 UV, X-rays UV d d, m h d m f, m g d c, h d, h, m, n c d, h, m, n d, h, m, n c, h, i i e, h, j, m, n c h d,j d, h, m, n k h, l d, k, m, n e, h, m °A representative listing of strains with multiple markers can be found in D.
E. Nucleic Acids . . . . . . . . . . . . . . . . . . . . . . . . . . . F. I n Vitro Amino Acid Incorporating System . . . . . . . . . . . . . . Antibiotics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Analogs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36 38 39 42 43 45 45 45 49 49 50 51 51 53 53 54 54 55 55 56 56 57 57 59 I.
A. Mutagenic Techniques Used Primarily in Vivo . . . . . . . . . . . . B. Mutagenic Techniques Used Primarily in Vitro . . . . . . . . . . . . C. Relative Effectiveness o f Mutagenic Treatments . . . . . . . . . . . Genetic Techniques . . . . . . . . . . . . . . . . . . . . . . . . . A. Transformation . . . . . . . . . . . . . . . . . . . . . . . . . B. Transduction . . . . . . . . . . . . .